In Africa, women are one of the continents greatest potentials to unlock economic growth. This is so given that they provide the majority of labour. Unfortunately, a traditionally male-dominated business environment in Africa is a barrier for most women to fully participate in the economy of their countries. Despite these challenges, a Ugandan woman has developed an entrepreneurial spirit and set up a successful business idea, showing that women have the capacity to succeed in business. Julian Adyeri Omalla has built a multi-million dollar drinks and food business and is the owner of Ugandas largest juice processing factory, Delight Uganda Limited. With her strong determination and leadership, Julian is one of the major employers in Uganda and is also coordinating womens farmer cooperatives. With an annual turnover of US$4million, her company owns 45% of the local market share for fruit juice. This story shows how women can overcome the gender barrier and transform their societies by building successful business activities. However, there remain some key challenges that require policy and decision makers attention in order to allow women to start and expand their businesses. The case study reveals the business opportunity that exists in the agribusiness sector in Africa and further discusses the persistent gender gap in African societies. It is recommended that gender gap is addressed adequately, for example, through better financial inclusion of women. In line with this, there is a need to build the capacities of African governments, to enable them to enter into strategic public-private partnerships that will mobilize more financial resources for female entrepreneurs. It is also important to develop capacity building programmes for women to equip them so that they can be able to capture financial resources and manage them efficiently. This case study further suggests the creation of agribusiness incubators to support female entrepreneurs from the idea stage to its implementation.